All sets are analyzed for cross compatibility and cross homology to other targets to ensure specific and efficient amplification.
PrimerPlex uses innovative and proprietary algorithms to design optimal primer sets, minimizing Tm mismatches, for up to 100 targets for working in a single tube multiplex assays. The results are automatically interpreted and the homologies identified are avoided during probe design, ensuring that the design is highly specific.ĭesigning multiplex primer sets that work well can be a challenging task. The program BLAST searches multiple sequences in a single search run. With PrimerPlex, you can design ASPE primers with the SNP at the 3' end and capture probes for direct hybridization assays. The oligos are screened for dimers, runs and repeats. The program not only designs oligos for detecting for the sequences, but enables you to design their corresponding amplifying primer pairs as well. The probes for both the assays are screened for cross homologies to ensure high signal strength. Using PrimerPlex, design oligos for ASPE and direct hybridization assays. In addition, PrimerPlex presents a list of alternate sets for you to choose from, based on your specific experimental needs. In the process, it analyzes millions of possible multiplex sets in a flash. PrimerPlex checks the oligos for cross homologies and minimizes Tm mismatches to give you the best possible multiplex set. The oligos are designed after avoiding the regions of homology identified by a BLAST search. PrimerPlex designs specific capture probes for direct hybridization assays and primers for Allele Specific PCR Extension (ASPE), for multiplexed high throughput SNP genotyping applications such as SNP analysis, pathogen detection, strain typing, and haplotyping. Based on Luminex Corporation's xMAP technology, these systems offer a versatile platform for multiplex nucleic acid detection in a 96-well format. PrimerPlex is an efficient and sophisticated tool for designing multiplex oligos for multiplex analysis platforms such as Luminex 100, Luminex 200, Bio-Plex 200 suspension array systems.
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